Applied biosystems identifiler plus user manual pdf

Ampflstr identifiler plus pcr amplification kit user guide pub. Manuals and user guides for applied biosystems steponeplus. L of the amplified product or allelic ladder and 0. Fitpro fit test software user manual hp vl420dt manual pdf file name size manual of exalted power sidereals pdf 99 mb. Str identifiler pcr amplification kit users manual. Genetic diversity of 15 autosomal short tandem repeats.

To verify your client connection to the database, you need a valid oracle user id and password. Str identifiler plus pcr amplification kit user guide publication number 4440211 rev. Portable document format pdf versions of this guide and the documents listed. Introduction the a15 analyzer is an automatic random access analyser specially designed for performing biochemical and turbi dimetric clinical analyses. Weider home gym user manual volvo truck manual pdf. The instrument is controlled online in real time from an external dedicated pc. Pcr products were separated and detected on an abi 3500 genetic analyzer using a 36 cm array and pop4 polymer applied biosystems. A comparison of ampflstr identifiler kit versus ampflstr. Applied biosystems 2001 ampflstr identifiler pcr amplification kit users manual.

For internal distri bution only february 7, 2003 11. Email promega technical services if you have questions on use of this system. Users manual 4309589 table 4 applied biosystems documents continued document part number. Reduction of artifacts in applied biosystems continual efforts to improve the quality of our products, we have made some modifi.

Within injections, the data shows no markers andor alleles with high standard deviation values exceeding 0. Amplification kits globalfiler and globalfiler iqc pcr. Ampflstr yfiler pcr amplification kit users manual pn 4358101c. Pdf 1 avoid here maps i ve only used it 2 or 3 times in the last couple of weeks now you 8217 re just waiting patiently for people to come, hoping that all that hard work will pay off. Ampflstr profiler plus kit amplification chapter 5. Applied biosystems 37303730 xl our scientific research. Profiler plus, cofiler, and sgm plus kits may quickly adopt the identifiler kit with complete confidence. Nfstc dna training workshop literature amplification. Dna casework technical procedures table of contents. Identifiler kit developmental validation 3 using the quantiblotr human dna quantitation kit applied biosystems or were ampf str control dna from the pro. Visit the web site to verify that you are using the most current version of this technical manual. Due to 5 dye chemistry of identifiler plus the loci are spread more evenly eliminating the visual merging of loci as can happen in powerplex 16 hs if you arent used to it.

Documents document view page applied biosystems 2720. This is the user guide for the ampflstr identifiler plus pcr amplification kit. Applied biosystems identifiler plus user manual samsung l760 user manual machine user manual pdf. The applied biosystems high resolution melting hrm software is the easiest to use melting analysis software enabling realtime pcr melt curve assays to be used more accurately for mutation scanning and genotyping. Journal of the association for laboratory automation 2016 10. As per the manufacturers instructions 12, dna amplification using the identifiler standard. Applied biosystems provides preformulated, readytouse, qualitytested 5 nuclease taqman assays for use with the stepone and steponeplus realtime pcr systems, as well as for all other realtime pcr platforms in the applied biosystems product portfolio table 4. All samples were analyzed using genemapper id software version 3.

Powerplexr fusion system for use on the applied biosystems. Automated forensic dna purification optimized for fta card. Biosystems offers a broad, extensive portfolio of clinical diagnostics products to streamline the work flow, enhance operational efficiency and improve patient treatment. Development of a fast pcr protocol enabling rapid generation. Ampfistr profiler plus pcr amplification kit users manual pn. Each word implies a particular level of observation or action as described below. Nist validation studies on the 3500 genetic analyzer. Applied biosystems 7500 and 7500 fast software version 2. Provides information that may be of interest or help but is not critical to the use of the product. The 16 loci amplified in this study are d3s58, vwa, fga, d8s1179, d21s11, d18s51, d5s818, ds317, d7s820, th01, tpox, csf1po, d19s433, d2s38, d16s539 and. Str data for the 15 ampflstr identifiler loci in the moroccan. Applied biosystems 2004a ampflstr identifiler pcr amplification kit.

Bowflex ultimate users manual download bowflex ultimate users manual. Ampflstr yfiler pcr amplification kit users manual iii. Since we pioneered realtime pcr over a decade ago, applied biosystems has continued to develop the technology to provide more powerful solutions to labs of all sizes. The globalfiler pcr amplification kit is the first 6dye, 24locus str kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic dna labs worldwide to maximize information recovery and improve overall efficiency. Ampflstr identifiler pcr amplification kit user guide pn. The ampflstr ngm pcr kit is a high discriminatory short tandem. For example, sample preparations and electrophoresis on the applied biosystems 3xl analyzer occurred as follows. Automated forensic dna purification optimized for fta card punches and identifiler strbased pcr analysis lois c. The identifiler plus users guide also allows for an increased sensitivity option. Comparison of identifiler, identifiler plus and minifiler. The applied biosystems ampflstr identifiler pcr amplification kit user. Licensing at applied biosystems, 850 lincoln centre drive, foster city, california 94404, or the licensing department, roche molecular systems, inc. Travel guide 2007 maldives pdf 74 mb ccd camera user manual 212 mb abb king pro manual pdf download 51 mb.

Precision studies using the abi prism 3100 genetic analyzer have been conducted by performing multiple runs of the ampf. Applied biosystems stepone steponeplus realtime pcr systems. Str identifiler plus pcr amplification kit product bulletin publication number 112pb3002 co11504 0411. The pcr amplification reaction mixture was prepared in a volume of 25 ll containing 10 llofampfstr identifiler plus master mix, 5 llof ampfstr identifiler plus primer set, and a. Applied biosystems 7900ht fast realtime pcr system and sds. When a hazard symbol and hazard type appear by a chemical name or instrument hazard, see the safety appendix for.

Abi prism, ampflstr, genotyper, microamp, profiler, and profiler plus are registered. Bowflex ultimate users manual downloadbowflex ultimate users manual. Print bookmark share pdf 350kb english format file size language download get adobe reader. Each word implies a particular level of observation. The ampflstr identifiler pcr amplification kit is the single most discriminating and widely used str based kit for human identification applications from applied biosystems. Validation experiments to evaluate the performance of this ce consisted of a precision study, sensitivity study, genotype concordance, a two person mixture study, and signal normalization. We have 1 applied biosystems steponeplus manual available for free pdf download. Installation, networking, and maintenance applied biosystems steponeplus installation, networking, and maintenance 184 pages. The various product lines include a wide range of reliable instruments and reagents for clinical chemistry, coagulation, hematology, autoimmunity and serology. The identifiler kit amplifies 15 loci and amelogenin in a single tube and provides loci consistent with major worldwide str databasing standards. Applied biosystems abi applied biosystems pcr applied biosystems 2720 thermal cycler tags.

Developmental validation of the ampfstr identifiler plus pcr. Dna in a geneamp 9700 pcr system applied biosystems, usa. Applied biosystems stepone and steponeplus realtime pcr systems reagent guide vii preface how to use this guide about the system documentation the guides listed below are shipped with the applied biosystems stepone and steponeplus realtime pcr systems stepone and steponeplus systems. Chapters in the ampflstr profiler plus pcr amplification kit users manual that are especially useful for the profiler plus id kit are as follows. An internal validation of the applied biosystems 8capillary 3500 genetic analyzer was performed using two commercial short tandem repeat str multiplex kits identifiler and identifiler plus. Ampflstr profiler plus id pcr amplification kit page 5 of 32 safety documentation user attention words five user attention words appear in the text of all applied biosystems user documentation. F 03 october 2019 add information for the globalfiler iqc pcr amplification kit. Specifically designed with a user friendly, yet powerful interface, they are ideal for.

Applied biosystems 7500 fast, 7500 and 7300 realtime pcr. Ampflstr identifiler pcr amplification kit user guide. Life technologies released the identifiler plus pcr amplification kit as an. Applied biosystems 2720 thermal cycler user manual. Str minifiler pcr amplification kit was designed to. Intrarun and interrun precision data demonstrates the sizing reproducibility of the 3100 instrument for forensic applications. Str identifiler kit identifiler, applied biosytems, foster city. Identifiler plus exhibited better heterozygote peak balance and mixture elucidation. The reagents are designed for use with the following applied biosystems instruments.

Across injections, the data indicates that high standard deviation. Forensic evaluation of the ampflstr ngm loci in lodz region of. Validation experiments to evaluate the performance of this ce consisted of a precision study, sensitivity study, genotype concordance, a two person mixture study, and signal normalization evaluation. Life technologiesapplied biosystems globalfiler 6dye liz600 size standard fga a vwa d18s51 d19s433 d8s1179 d21s11 d3s58 th01 d16s539 d2s38 d22s1045 d2s441 se33 100 bp 200 bp 300 bp 400 bp d10s1248 d1s1656 d12s391 d5s818 ds317 d7s820 csf1po y dys391 tpox 24plex 6fam vic ned taz sid. Pdf populationgenetic and comparative interpopulation. Overall success in obtaining interpretable dna profiles was 15. Applied biosystems 1998 abi prismtm genescan analysis software 3. Pdf, ebook, free books to download, user guard, ebook, books, manual, bowflex, ultimate, users, manual. Duo dna quantification kit applied biosystems on the abi. The identifiler kit uses a fivedye fluorescent system for automated dna fragment analysis.

Applied biosystems pet and liz dyes expand the spectral detection range that can be used on abi prism genetic. The ampflstr profiler plus pcr amplification kit users manual pn 4303501 should be referred to for use with the ampflstr profiler plus id kit. Primate dna samples were obtained from bios laboratories, inc. Powerplex 16 hs proved more sensitive than identifiler plus. Applied biosystems 37303730xl dna analyzers user guide ix general safety documentation user attention words five user attention words appear in the text of all applied biosystems user documentation.

To learn more about reagents, consumables and assays, please visit info. Applied biosystems quantstudio 6 flex realtime pcr system. Development of a fast pcr protocol enabling rapid generation of. Ampflstr identifiler plus user guide 7 preface revision history purpose the applied biosystems ampflstr identifiler plus pcr amplification kit user guide provides information about the applied biosystems instruments, chemistries, and software associated with the ampflstr identifiler plus pcr amplification kit. Applied biosystems quantstudio 6 flex realtime pcr system, 96well fast, laptop facilitates realtime pcr based applications with an upgradable block selection, allowing you to plan for experiments today and grow with tomorrows needs. Ampflstr identifilertm users manual, applied biosystems, usa, 2001. Ab design, abi prism, ampflstr, applied biosystems, cofiler, genotyper, liz, microamp, pet, and vic are. Ampflstr identifiler plus pcr amplification kit user guide. View online or download applied biosystems 7500 installation and maintenance manual, getting started, getting started manual. Identifiler and identifiler plus thermal cycling protocols followed manufacturer recommendations found in each kits manual, and was carried out on the geneamp 9700 thermal cycler applied biosystems. The applied biosystems ampf lstr identifiler plus pcr amplification kit user guide provides information about the applied biosystems instruments, chemistries. Save target as to download a pdf version of the document to a.

Str identifiler allelic ladder, 9947a kit positive control dna and the 250basepair fragment from the internal size standard genescan liz500. Simultaneous amplifications of 16 str loci multiplexed pcr were performed by using the ampflstr identifiler pcr amplification kit according to the users manual recommendations. Precision studies using the abi prism 3100 genetic. The globalfiler pcr amplification kit is the first 6dye, 24locus str kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic dna labs worldwide to maximize information. Applied biosystems, abi prism, ampflstr, cofiler, genescan, geno typer. Applied biosystems offers a family of realtime pcr instruments with the 96well block format. In each of the elements of the a15 analyser, biosystems has used leading edge technology to obtain optimum ana. For general safety information, see this preface and appendix b, safety on page 122. The applied biosystems 7500 fast realtime pcr system represents the latest. Safety alert words safety alert words also appear in user. Guide provides information about the applied biosystems instruments.

The pcr amplification reaction mixture was prepared in a volume of 25 ll containing 10 llofampfstr identifiler plus master mix, 5 llof ampfstr identifiler plus primer set, and a maximum volume of 10 ll of target dna. Applied biosystems 7900ht fast realtime pcr system and. Allele frequencies for the 15 str loci included in the ampflstr identifiler. Ampfistr sefiler plus pcr amplification kit user guide pn. Profiler plus user bulletin san jose state university. Str data for the 15 ampflstr identifiler loci in the. The genetic diversity and forensic parameters based on 15 autosomal short tandem repeats str loci.

The applied biosystems ampflstr identifiler plus pcr amplification kit user. Precision studies using the abi prism 3100 genetic analyzer. By adding an additional dye, more loci can be multiplexed in a single pcr amplification as compared to the previous 4dye system. Pullout chapters this guide is designed to allow users to pull out chapters 2, 3, and 4. These improvements enhance the assay signaltonoise ratio and. The hrm software is available on the 7500 fast realtime pcr system, which delivers precise results in a standard 96well format. Two user attention words appear in applied biosystems user documentation. C thermal cycler instruction manual, 10010305 rev b. Specifically designed with a user friendly, yet powerful interface, they are ideal for both new and experienced realtime pcr researchers. The identifiler kit contains all the necessary reagents for the amplification of human genomic dna. The identifiler kit amplifies 15 loci and amelogenin in a single tube and provides loci consistent with major worldwide str d. Unless stated otherwise, the protocols in the identifiler plus kit user guide were followed 15. In the event that the instrument has been subjected to adverse environmental conditions such as. Applied biosystems stepone steponeplus realtime pcr.